Mouse Myelin protein zero-like protein 2 (MPZL2) ELISA Kit | KTE70959

Mouse Myelin protein zero-like protein 2 (MPZL2) ELISA Kit | KTE70959 | Gentaur UK, US & Europe Distribution

Application: This Mouse Myelin protein zero-like protein 2 (MPZL2) ELISA Kit employs a two-site sandwich ELISA to quantitate MPZL2 in samples. An antibody specific for MPZL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMPZL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MPZL2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MPZL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Myelin protein zero-like protein 2 microplate
• Mouse Myelin protein zero-like protein 2 standard
• Mouse Myelin protein zero-like protein 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Myelin protein zero-like protein 2 (MPZL2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MPZL2. No significant cross-reactivity or interference between Mouse MPZL2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Thymus development depends on a complex series of interactions between thymocytes and the stromal component of the organ. Epithelial V-like antigen (EVA) is expressed in thymus epithelium and strongly downregulated by thymocyte developmental progression. Myelin protein zero-like protein 2 (MPZL2) is expressed in the thymus and in several epithelial structures early in embryogenesis. It is highly homologous to the myelin protein zero and, in thymus-derived epithelial cell lines, is poorly soluble in nonionic detergents, strongly suggesting an association to the cytoskeleton. Its capacity to mediate cell adhesion through a homophilic interaction and its selective regulation by T cell maturation might imply the participation of EVA in the earliest phases of thymus organogenesis. MPZL2 bears a characteristic V-type domain and two potential N-glycosylation sites in the extracellular domain; a putative serine phosphorylation site for casein kinase 2 is also present in the cytoplasmic tail. Two transcript variants encoding the same protein have been found for MPZL2.

Alternative Names: MPZL2; EVA; EVA1; epithelial V-like antigen 1

Search name: MPZL2; EVA; EVA1; epithelial V-like antigen 1

Tag: MPZL2