Mouse Paired mesoderm homeobox protein 2 (PRRX2) ELISA Kit | KTE70644

Mouse Paired mesoderm homeobox protein 2 (PRRX2) ELISA Kit | KTE70644 | Gentaur UK, US & Europe Distribution

Application: This Mouse Paired mesoderm homeobox protein 2 (PRRX2) ELISA Kit employs a two-site sandwich ELISA to quantitate PRRX2 in samples. An antibody specific for PRRX2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRRX2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRRX2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRRX2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Paired mesoderm homeobox protein 2 microplate
• Mouse Paired mesoderm homeobox protein 2 standard
• Mouse Paired mesoderm homeobox protein 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Paired mesoderm homeobox protein 2 (PRRX2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PRRX2. No significant cross-reactivity or interference between Mouse PRRX2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: PRRX2 is a member of the paired family of homeobox proteins. Expression is localized to proliferating fetal fibroblasts and the developing dermal layer, with downregulated expression in adult skin. Increases in expression of this gene during fetal but not adult wound healing suggest a possible role in mechanisms that control mammalian dermal regeneration and prevent formation of scar response to wounding. The composite 813-nucleotide cDNA encodes a putative 164-amino acid protein with 74% overall identity to mouse Prx2, showing high conservation in the homeodomain and extreme 3-prime region. PRX2 is expressed as a single 1.3-kb transcript. PRX2 is more strongly expressed in normal fetal than in adult skin, much more strongly expressed in both fetal and adult proliferating fibroblasts, and not expressed in keratinocytes.

Alternative Names: PRRX2; MGC19843; PMX2; PRX2; paired mesoderm homeobox protein 2; paired-like homeodomain protein PRX2

Search name: PRRX2; MGC19843; PMX2; PRX2; paired mesoderm homeobox protein 2; paired-like homeodomain protein PRX2

Tag: PRRX2