Mouse PARP2 (Poly [ADP-ribose] polymerase 2) ELISA Kit | ELK8380

Mouse PARP2 (Poly [ADP-ribose] polymerase 2) ELISA Kit | ELK8380 | Gentaur UK, US & Europe Distribution

Alternative Names: Poly [ADP-ribose] polymerase 2 (PARP-2) (hPARP-2) (EC 2.4.2.30) (ADP-ribosyltransferase diphtheria toxin-like 2) (ARTD2) (NAD (+) ADP-ribosyltransferase 2) (ADPRT-2) (Poly[ADP-ribose] synthase 2) (pADPRT-2), PARP2, ADPRT2 ADPRTL2

Species: Mouse

Assay Type: Sandwich

Sensitivity: 7.2 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: DNA damage/DNA repair

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Poly [ADP-ribose] polymerase 2 (Mouse PARP2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Poly [ADP-ribose] polymerase 2 (Mouse PARP2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Poly [ADP-ribose] polymerase 2 (Mouse PARP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Poly [ADP-ribose] polymerase 2 (Mouse PARP2) in the samples is then determined by comparing the OD of the samples to the standard curve.