Mouse Patatin-like phospholipase domain-containing protein 2 (PNPLA2) ELISA Kit | AE26776MO

Mouse Patatin-like phospholipase domain-containing protein 2 (PNPLA2) ELISA Kit | AE26776MO | Gentaur UK, US & Europe Distribution

Species Reactivity: Mouse (Mus musculus)

Abbreviation: PNPLA2

Alternative Name: 1110001C14Rik; ATGL; DESNUTRIN; DKFZp667M109; FP17548; PEDF-R; TTS-2.2; TTS2; adipose triglyceride lipase|pigment epithelium-derived factor|transport-secretion protein 2.2|triglyceride hydrolase

Application: ELISA

Range: 0.312-20 ng/mL

Sensitivity: 0.128 ng/mL

Intra-Assay: ≤5.1%

Inter-Assay: ≤8.1%

Recovery: 0, 89

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate PNPLA2 in samples. An antibody specific for PNPLA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPNPLA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PNPLA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PNPLA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: The deduced 486-amino acid protein contains a highly conserved 180-amino acid N-terminal patatin domain with a glycine-rich region, an active serine hydrolase motif, and an aspartate active site motif. PNPLA2, which shows 88% amino acid homology to mouse desnutrin. Northern blot analysis detected a 2.0-kb transcript expressed at high levels in mouse brown and white adipose tissue and at intermediate levels in heart and testis. Very low expression was detected in all other mouse tissues examined. Expression of desnutrin was lower in gonadal fat deposits of genetically obese mice compared with wildtype mice. Desnutrin mRNA was found only in mature adipocytes and not in proliferating or confluent mouse preadipocytes. Expression was rapidly increased in cells induced to differentiate.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .