Mouse PDIA2 (Protein Disulfide Isomerase A2) ELISA Kit | ELK3306

Mouse PDIA2 (Protein Disulfide Isomerase A2) ELISA Kit | ELK3306 | Gentaur UK, US & Europe Distribution

Alternative Names: PDI-A2; PDA2, PDI, PDIR; PDIP; Protein Disulfide Isomerase, Pancreatic; Protein Disulfide Isomerase-Associated 2; Pancreas-specific protein disulfide isomerase

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.064 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Metabolic pathway;Hepatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Disulfide Isomerase A2 (PDIA2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Protein Disulfide Isomerase A2 (PDIA2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Disulfide Isomerase A2 (PDIA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Disulfide Isomerase A2 (PDIA2) in the samples is then determined by comparing the OD of the samples to the standard curve.