Mouse Peptidoglycan recognition protein 1 (PGLYRP1) ELISA Kit | KTE70761

Mouse Peptidoglycan recognition protein 1 (PGLYRP1) ELISA Kit | KTE70761 | Gentaur UK, US & Europe Distribution

Application: This Mouse Peptidoglycan recognition protein 1 (PGLYRP1) ELISA Kit employs a two-site sandwich ELISA to quantitate PGLYRP1 in samples. An antibody specific for PGLYRP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPGLYRP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGLYRP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGLYRP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Peptidoglycan recognition protein 1 microplate
• Mouse Peptidoglycan recognition protein 1 standard
• Mouse Peptidoglycan recognition protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Peptidoglycan recognition protein 1 (PGLYRP1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PGLYRP1. No significant cross-reactivity or interference between Mouse PGLYRP1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Innate nonself immune recognition relies on structures common among invading microbes, a process termed pattern recognition. Peptidoglycan is a fundamental component of the bacterial cell wall and is thus a candidate for a pattern recognized by the immune system. The deduced human PGLYRP protein has 196 amino acids. Both the human and mouse PGLYRP proteins share 43% sequence identity with the T. ni PGLYRP protein. Recombinant mouse Pglyrp protein expressed in insect cells possessed an affinity for peptidoglycan. Dot blot analysis of a number of human tissue mRNAs detected strong expression in bone marrow and weak expression in lung, kidney, liver, small intestine, spleen, thymus, peripheral leukocyte, and fetal spleen.

Alternative Names: PGLYRP1; MGC126894; MGC126896; PGLYRP; PGRP; PGRP-S; PGRPS; TAG7; TNFSF3L; TNF superfamily; member 3 (LTB) -like (peptidoglycan recognition protein)

Search name: PGLYRP1; MGC126894; MGC126896; PGLYRP; PGRP; PGRP-S; PGRPS; TAG7; TNFSF3L; TNF superfamily; member 3 (LTB) -like (peptidoglycan recognition protein)

Tag: PGLYRP1