Mouse Periplakin (PPL) ELISA Kit | KTE70706

Mouse Periplakin (PPL) ELISA Kit | KTE70706 | Gentaur UK, US & Europe Distribution

Application: This Mouse Periplakin (PPL) ELISA Kit employs a two-site sandwich ELISA to quantitate PPL in samples. An antibody specific for PPL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPL bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Periplakin microplate
• Mouse Periplakin standard
• Mouse Periplakin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Periplakin (PPL) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PPL. No significant cross-reactivity or interference between Mouse PPL and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Periplakin is a component of desmosomes and of the epidermal cornified envelope in keratinocytes. The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments. This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments. AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1-mediated signaling.The deduced 1, 666-amino acid protein has a calculated molecular mass of about 205 kD. PPL shares extensive sequence homology with other proteins of the plakin family, with highest identities found in the N- and C-terminal domains.

Alternative Names: PPL; KIAA0568; MGC134872; 190 kDa paraneoplastic pemphigus antigen; 195 kDa cornified envelope

Search name: PPL; KIAA0568; MGC134872; 190 kDa paraneoplastic pemphigus antigen; 195 kDa cornified envelope

Tag: PPL