Mouse Peroxisomal testis-specific protein 1 (PXT1) ELISA Kit | KTE70597

Mouse Peroxisomal testis-specific protein 1 (PXT1) ELISA Kit | KTE70597 | Gentaur UK, US & Europe Distribution

Application: This Mouse Peroxisomal testis-specific protein 1 (PXT1) ELISA Kit employs a two-site sandwich ELISA to quantitate PXT1 in samples. An antibody specific for PXT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPXT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PXT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PXT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Peroxisomal testis-specific protein 1 microplate
• Mouse Peroxisomal testis-specific protein 1 standard
• Mouse Peroxisomal testis-specific protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Peroxisomal testis-specific protein 1 (PXT1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PXT1. No significant cross-reactivity or interference between Mouse PXT1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Genes reported to be crucial for spermatogenesis are often exclusively expressed in the testis. The putative amino acid sequence encoded by the cDNA of the Pxt1 gene contains a conserved Asn-His-Leu (NHL) -motif at its C-terminal end, which is characteristic for peroxisomal proteins. Pxt1-EGFP fusion protein is co-localized with known peroxisomal marker proteins in transfected NIH3T3 cells. The peroxisomal targeting signal NHL is functional and responsible for the correct subcellular localization of the Pxt1-EGFP fusion protein. In male germ cells peroxisomes were reported only in spermatogonia. The Pxt1 gene is so far the first gene coding for a putative peroxisomal protein which is expressed in later steps of spermatogenesis, namely in pachytene spermatocytes.

Alternative Names: PXT1; MGC129569; STEPP; small testis-specific peroxisomal protein

Search name: PXT1; MGC129569; STEPP; small testis-specific peroxisomal protein

Tag: PXT1