Mouse PHF8 (PHD Finger Protein 8) ELISA Kit | ELK6954

Mouse PHF8 (PHD Finger Protein 8) ELISA Kit | ELK6954 | Gentaur UK, US & Europe Distribution

Alternative Names: ZNF422; MRXSSD; JHDM1F; Jumonji C Domain-Containing Histone Demethylase 1F; Histone lysine demethylase PHF8

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.118 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PHD Finger Protein 8 (PHF8) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PHD Finger Protein 8 (PHF8) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PHD Finger Protein 8 (PHF8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PHD Finger Protein 8 (PHF8) in the samples is then determined by comparing the OD of the samples to the standard curve.