Mouse Plastin-1 (PLS1) ELISA Kit | KTE70725

Mouse Plastin-1 (PLS1) ELISA Kit | KTE70725 | Gentaur UK, US & Europe Distribution

Application: This Mouse Plastin-1 (PLS1) ELISA Kit employs a two-site sandwich ELISA to quantitate PLS1 in samples. An antibody specific for PLS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Plastin-1 microplate
• Mouse Plastin-1 standard
• Mouse Plastin-1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Plastin-1 (PLS1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PLS1. No significant cross-reactivity or interference between Mouse PLS1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. The protein encoded by this gene is a third distinct plastin isoform, which is specifically expressed at high levels in the small intestine. Alternatively spliced transcript variants varying in the 5' UTR, but encoding the same protein, have been found for this gene. A pseudogene of this gene is found on chromosome 11. The predicted 629-amino acid protein is 86%, 75%, and 73% identical to chicken fimbrin, human T-plastin (PLS3), and L-plastin (LCP1), respectively. I-plastin migrated as a 68-kD protein on Western blots of human small intestine and colon extracts.

Alternative Names: PLS1; FIMBRIN; Plastin-1; intestine-specific plastin; plastin 1

Search name: PLS1; FIMBRIN; Plastin-1; intestine-specific plastin; plastin 1

Tag: PLS1