Mouse Plexin-A1 (PLXNA1) ELISA Kit | KTE70723

Mouse Plexin-A1 (PLXNA1) ELISA Kit | KTE70723 | Gentaur UK, US & Europe Distribution

Application: This Mouse Plexin-A1 (PLXNA1) ELISA Kit employs a two-site sandwich ELISA to quantitate PLXNA1 in samples. An antibody specific for PLXNA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLXNA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLXNA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLXNA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Plexin-A1 microplate
• Mouse Plexin-A1 standard
• Mouse Plexin-A1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Plexin-A1 (PLXNA1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PLXNA1. No significant cross-reactivity or interference between Mouse PLXNA1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: PLXN1 alone did not bind semaphorin-3A (SEMA3A), but the NRP1/PLXN1 complex had a higher affinity for SEMA3A than did NRP1 alone. While SEMA3A binding to NRP1 did not alter nonneuronal cell morphology, SEMA3A interaction with NRP1/PLXN1 complexes induced adherent cells to round up. Expression of a dominant-negative PLXN1 in sensory neurons blocked SEMA3A-induced growth cone collapse. SEMA3A treatment led to the redistribution of growth cone NRP1 and PLXN1 into clusters. Thus, the authors concluded that physiologic SEMA3A receptors consist of NRP1/PLXN1 complexes.The transmembrane protein they called NOV was identified by a cDNA found in a skeletal muscle cDNA library and shares 72% identity with SEX.

Alternative Names: PLXNA1; DKFZp761P19121; NOV; NOVP; PLEXIN-A1; PLXN1; plexin 1

Search name: PLXNA1; DKFZp761P19121; NOV; NOVP; PLEXIN-A1; PLXN1; plexin 1

Tag: PLXNA1