Mouse PLGF (Placenta Growth Factor) ELISA Kit | ELK1242

Mouse PLGF (Placenta Growth Factor) ELISA Kit | ELK1242 | Gentaur UK, US & Europe Distribution

Alternative Names: PlGF2; PGF; PGFL; Placental Growth Factor-Like; Vascular Endothelial Growth Factor-Related Protein

Species: Mouse

Assay Type: Sandwich

Sensitivity: 12.1 pg/mL

Standard: 500 pg/mL

Detection Range: 7.82-500 pg/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Cytokine;Reproductive science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Placenta Growth Factor (PLGF) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Placenta Growth Factor (PLGF) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Placenta Growth Factor (PLGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Placenta Growth Factor (PLGF) in the samples is then determined by comparing the OD of the samples to the standard curve.