Mouse PPL (Periplakin) ELISA Kit | ELK6363

Mouse PPL (Periplakin) ELISA Kit | ELK6363 | Gentaur UK, US & Europe Distribution

Alternative Names: 190 kDa paraneoplastic pemphigus antigen; 195 kDa cornified envelope precursor protein

Species: Mouse

Assay Type: Sandwich

Sensitivity: 14 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Periplakin (PPL) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Periplakin (PPL) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Periplakin (PPL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Periplakin (PPL) in the samples is then determined by comparing the OD of the samples to the standard curve.