Mouse PPP3R1 (Protein Phosphatase 3, Regulatory Subunit 1) ELISA Kit | ELK6060

Mouse PPP3R1 (Protein Phosphatase 3, Regulatory Subunit 1) ELISA Kit | ELK6060 | Gentaur UK, US & Europe Distribution

Alternative Names: CALNB1; CNB; CNB1; Calcineurin B Type I; Protein phosphatase 2B regulatory subunit 1; Protein phosphatase 3 regulatory subunit B alpha isoform 1

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.047 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) in the samples is then determined by comparing the OD of the samples to the standard curve.