Mouse PRELI domain-containing protein 1, mitochondrial (PRELID1) ELISA Kit | KTE70690

Mouse PRELI domain-containing protein 1, mitochondrial (PRELID1) ELISA Kit | KTE70690 | Gentaur UK, US & Europe Distribution

Application: This Mouse PRELI domain-containing protein 1, mitochondrial (PRELID1) ELISA Kit employs a two-site sandwich ELISA to quantitate PRELID1 in samples. An antibody specific for PRELID1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRELID1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRELID1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRELID1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse PRELI domain-containing protein 1, mitochondrial microplate
• Mouse PRELI domain-containing protein 1, mitochondrial standard
• Mouse PRELI domain-containing protein 1, mitochondrial detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse PRELI domain-containing protein 1, mitochondrial (PRELID1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PRELID1. No significant cross-reactivity or interference between Mouse PRELID1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The predicted 219-amino acid human protein is 85% identical to the avian protein. PX19 contains 2 alpha helices with amphipathic domains in opposite orientation, and the LEA motifs (A/TAEKAK) are clustered within this region. Northern blot analysis detected 1.0- and 1.2-kb transcripts predominantly in fetal liver, with much lower levels in other fetal tissues. Expression was significantly reduced in adult liver, but was higher in other adult organs. Expression was low in bone marrow and thymus, but high in adult spleen, lymph node, and peripheral blood leukocytes. Further analysis of B-cell lines indicated that expression was higher in mature B cells, predominantly from germinal centers, and absent in progenitor and precursor B-cell lines.

Alternative Names: PRELID1; CGI-106; MGC87972; PRELI; PX19; protein of relevant evolutionary and lymphoid interest; px19-like protein

Search name: PRELID1; CGI-106; MGC87972; PRELI; PX19; protein of relevant evolutionary and lymphoid interest; px19-like protein

Tag: PRELID1