Mouse PRKAa1 (Protein Kinase, AMP Activated Alpha 1) ELISA Kit | ELK5105

Mouse PRKAa1 (Protein Kinase, AMP Activated Alpha 1) ELISA Kit | ELK5105 | Gentaur UK, US & Europe Distribution

Alternative Names: AMPK; AMPKa1; PKA; ACACA kinase; 5'-AMP-Activated Protein Kinase Catalytic Subunit Alpha-1; Acetyl-CoA carboxylase kinase; Hydroxymethylglutaryl-CoA reductase kinase

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.67 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase, AMP Activated Alpha 1 (PRKAa1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Protein Kinase, AMP Activated Alpha 1 (PRKAa1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase, AMP Activated Alpha 1 (PRKAa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase, AMP Activated Alpha 1 (PRKAa1) in the samples is then determined by comparing the OD of the samples to the standard curve.