Mouse Probable prolyl-tRNA synthetase, mitochondrial (PARS2) ELISA Kit | KTE71458

Mouse Probable prolyl-tRNA synthetase, mitochondrial (PARS2) ELISA Kit | KTE71458 | Gentaur UK, US & Europe Distribution

Application: This Mouse Probable prolyl-tRNA synthetase, mitochondrial (PARS2) ELISA Kit employs a two-site sandwich ELISA to quantitate PARS2 in samples. An antibody specific for PARS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPARS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PARS2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PARS2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Probable prolyl-tRNA synthetase, mitochondrial microplate
• Mouse Probable prolyl-tRNA synthetase, mitochondrial standard
• Mouse Probable prolyl-tRNA synthetase, mitochondrial detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Probable prolyl-tRNA synthetase, mitochondrial (PARS2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PARS2. No significant cross-reactivity or interference between Mouse PARS2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: By searching a database for tRNA synthetases, Bonnefond et al. (2005) identified PARS2, which they called MT-PRORS. The deduced 475-amino acid protein has a 47-amino acid mitochondrial targeting signal, resulting in a mature protein of 428 amino acids. PARS2 is a class II amino acid tRNA synthetase, with a C-terminal active-site domain linked to an N-terminal anticodon-binding domain by a short hinge. PARS2 shares no significant similarity with its cytosolic counterpart, EPRS. Bonnefond et al. (2005) determined that the PARS2 gene contains 1 exon and spans 1.4 kb. By genomic sequence analysis, Bonnefond et al. (2005) mapped the PARS2 gene to chromosome 1.

Alternative Names: PARS2; DKFZp727A071; MGC14416; MGC19467; MT-PRORS; proline tRNA ligase 2; mitochondrial (putative) ; prolyl-tRNA synthetase (mitochondrial) (putative) ; prolyl-tRNA synthetase; mitochondrial (putative)

Search name: PARS2; DKFZp727A071; MGC14416; MGC19467; MT-PRORS; proline tRNA ligase 2; mitochondrial (putative) ; prolyl-tRNA synthetase (mitochondrial) (putative) ; prolyl-tRNA synthetase; mitochondrial (putative)

Tag: PARS2