Mouse Protein naked cuticle homolog 1 (NKD1) ELISA Kit | KTE71463

Mouse Protein naked cuticle homolog 1 (NKD1) ELISA Kit | KTE71463 | Gentaur UK, US & Europe Distribution

Application: This Mouse Protein naked cuticle homolog 1 (NKD1) ELISA Kit employs a two-site sandwich ELISA to quantitate NKD1 in samples. An antibody specific for NKD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNKD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NKD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NKD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Protein naked cuticle homolog 1 microplate
• Mouse Protein naked cuticle homolog 1 standard
• Mouse Protein naked cuticle homolog 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Protein naked cuticle homolog 1 (NKD1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse NKD1. No significant cross-reactivity or interference between Mouse NKD1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: In the mouse, Nkd is a Dishevelled-binding protein that functions as a negative regulator of the Wnt-beta-catenin-Tcf signaling pathway. The deduced 470-amino acid protein contains an EF-hand Ca (2+) -binding motif. NKD1 shares about 44% overall identity with NKD2, including more than 75% identity in 4 major homology domains, including the EF-hand motif. Northern blot analysis revealed wide expression of 5 transcripts of 9.3 to 2.3 kb in adult and fetal tissues and in cancer cell lines. Highest expression was detected in fetal kidney, followed by fetal lung, placenta, and adult liver, kidney, pancreas, and spleen. Multiple transcripts were generated through the use of alternative polyadenylation sites.

Alternative Names: NKD1; Naked1; Dvl-binding protein; naked cuticle homolog 1; naked cuticle-1

Search name: NKD1; Naked1; Dvl-binding protein; naked cuticle homolog 1; naked cuticle-1

Tag: NKD1