Mouse Protein phosphatase 1B (PPM1B) ELISA Kit | KTE70705

Mouse Protein phosphatase 1B (PPM1B) ELISA Kit | KTE70705 | Gentaur UK, US & Europe Distribution

Application: This Mouse Protein phosphatase 1B (PPM1B) ELISA Kit employs a two-site sandwich ELISA to quantitate PPM1B in samples. An antibody specific for PPM1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPM1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPM1B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPM1B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Protein phosphatase 1B microplate
• Mouse Protein phosphatase 1B standard
• Mouse Protein phosphatase 1B detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Protein phosphatase 1B (PPM1B) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PPM1B. No significant cross-reactivity or interference between Mouse PPM1B and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: PPM1B is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase has been shown to dephosphorylate cyclin-dependent kinases (CDKs), and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to cause cell-growth arrest or cell death. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional transcript variants have been described, but currently do not represent full-length sequences. The deduced 479-amino acid protein shows high sequence similarity to other mammalian PP2C-beta isoforms but has an extended and unique C-terminal portion.

Alternative Names: PPM1B; MGC21657; PP2C-beta-X; PP2CB; PP2CBETA; PPC2BETAX; protein phosphatase 1B; protein phosphatase 2C-like protein

Search name: PPM1B; MGC21657; PP2C-beta-X; PP2CB; PP2CBETA; PPC2BETAX; protein phosphatase 1B; protein phosphatase 2C-like protein

Tag: PPM1B