Mouse Protein PML (PML) ELISA Kit | KTE70720

Mouse Protein PML (PML) ELISA Kit | KTE70720 | Gentaur UK, US & Europe Distribution

Application: This Mouse Protein PML (PML) ELISA Kit employs a two-site sandwich ELISA to quantitate PML in samples. An antibody specific for PML has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPML present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PML is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PML bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Protein PML microplate
• Mouse Protein PML standard
• Mouse Protein PML detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Protein PML (PML) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PML. No significant cross-reactivity or interference between Mouse PML and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Probable transcription factor PML is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies (Nuclear dots) where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL) . Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified.

Alternative Names: PML; MYL; PP8675; RNF71; TRIM19; promyelocytic leukemia protein; promyelocytic leukemia; inducer of; tripartite motif protein TRIM19

Search name: PML; MYL; PP8675; RNF71; TRIM19; promyelocytic leukemia protein; promyelocytic leukemia; inducer of; tripartite motif protein TRIM19

Tag: PML