Mouse Putative histone-lysine N-methyltransferase PRDM6 (PRDM6) ELISA Kit | KTE70697

Mouse Putative histone-lysine N-methyltransferase PRDM6 (PRDM6) ELISA Kit | KTE70697 | Gentaur UK, US & Europe Distribution

Application: This Mouse Putative histone-lysine N-methyltransferase PRDM6 (PRDM6) ELISA Kit employs a two-site sandwich ELISA to quantitate PRDM6 in samples. An antibody specific for PRDM6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRDM6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRDM6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRDM6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Putative histone-lysine N-methyltransferase PRDM6 microplate
• Mouse Putative histone-lysine N-methyltransferase PRDM6 standard
• Mouse Putative histone-lysine N-methyltransferase PRDM6 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Putative histone-lysine N-methyltransferase PRDM6 (PRDM6) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PRDM6. No significant cross-reactivity or interference between Mouse PRDM6 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: PRDM6, is enriched in flk1 (+) hematovascular precursor cells using a microarray-based approach. PRDM6 is also detected in embryonic and adult-derived endothelial cell lines. PRDM6 is co-localized with histone H4 and methylates H4-K20 (but not H3) in vitro and in vivo. Overexpression of PRDM6 in mouse embryonic endothelial cells induces apoptosis by activating caspase-3 and inducing G1 arrest. PRDM6 inhibits cell proliferation as determined by BrdU incorporation in endothelial cells, but not in rat aortic smooth muscle cells. PRDM6 is expressed by vascular precursors, has differential effects in endothelial cells and smooth muscle cells, and may play a role in vascular precursor differentiation and survival by modulating local chromatin-remodeling activity within hematovascular subpopulations during development.

Alternative Names: PRDM6; PR-domain zinc finger protein 6

Search name: PRDM6; PR-domain zinc finger protein 6

Tag: PRDM6