Mouse RAD51-associated protein 1 (RAD51AP1) ELISA Kit | KTE71428

Mouse RAD51-associated protein 1 (RAD51AP1) ELISA Kit | KTE71428 | Gentaur UK, US & Europe Distribution

Application: This Mouse RAD51-associated protein 1 (RAD51AP1) ELISA Kit employs a two-site sandwich ELISA to quantitate RAD51AP1 in samples. An antibody specific for RAD51AP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRAD51AP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAD51AP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAD51AP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse RAD51-associated protein 1 microplate
• Mouse RAD51-associated protein 1 standard
• Mouse RAD51-associated protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse RAD51-associated protein 1 (RAD51AP1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse RAD51AP1. No significant cross-reactivity or interference between Mouse RAD51AP1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: RAD51AP1 specifically stimulated joint molecule formation through structure-specific DNA binding and physical contact with RAD51. RAD51AP1 was required to protect HeLa cells from agents that cause DNA double-strand breaks. At the biochemical level, RAD51AP1 had selective affinity for branched DNA structures, and it stimulated D loop formation by RAD51, a pivotal step in recombination. The predicted PIR51 protein has 335 amino acids and a calculated pI of 9.95. The authors suggested that PIR51 is the human homolog of mouse Rab22 since the 2 proteins share 63% sequence identity. Northern blot analysis revealed that PIR51 is expressed as a 2.4-kb mRNA in human testis and thymus, and at lower levels, in colon and small intestine.

Alternative Names: RAD51AP1; PIR51; RAD51-interacting protein

Search name: RAD51AP1; PIR51; RAD51-interacting protein

Tag: RAD51AP1