Mouse RAPSN (Receptor Associated Protein Of The Synapse) ELISA Kit | ELK7236

Mouse RAPSN (Receptor Associated Protein Of The Synapse) ELISA Kit | ELK7236 | Gentaur UK, US & Europe Distribution

Alternative Names: CMS1D; CMS1E; RNF205; Rapsyn; Acetylcholine receptor-associated 43 kDa protein; 43 kDa postsynaptic protein; RING finger protein 205

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.55 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor Associated Protein Of The Synapse (RAPSN) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Receptor Associated Protein Of The Synapse (RAPSN) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Receptor Associated Protein Of The Synapse (RAPSN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Receptor Associated Protein Of The Synapse (RAPSN) in the samples is then determined by comparing the OD of the samples to the standard curve.