Mouse Receptor-type tyrosine-protein phosphatase T (PTPRT) ELISA Kit | KTE70612

Mouse Receptor-type tyrosine-protein phosphatase T (PTPRT) ELISA Kit | KTE70612 | Gentaur UK, US & Europe Distribution

Application: This Mouse Receptor-type tyrosine-protein phosphatase T (PTPRT) ELISA Kit employs a two-site sandwich ELISA to quantitate PTPRT in samples. An antibody specific for PTPRT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTPRT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTPRT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTPRT bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Receptor-type tyrosine-protein phosphatase T microplate
• Mouse Receptor-type tyrosine-protein phosphatase T standard
• Mouse Receptor-type tyrosine-protein phosphatase T detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Receptor-type tyrosine-protein phosphatase T (PTPRT) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PTPRT. No significant cross-reactivity or interference between Mouse PTPRT and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: T is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem intracellular catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains a meprin-A5 antigen-PTP (MAM) domain, Ig-like and fibronectin type III-like repeats. The protein domain structure and the expression pattern of the mouse counterpart of this PTP suggest its roles in both signal transduction and cellular adhesion in the central nervous system. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. Organism species: Homo sapiens (Human)

Alternative Names: PTPRT; RP5-1121H13.2; KIAA0283; RPTPrho; receptor protein tyrosine phosphatase

Search name: PTPRT; RP5-1121H13.2; KIAA0283; RPTPrho; receptor protein tyrosine phosphatase

Tag: PTPRT