(Mouse) Rnf2 Antibody (Center) [APR33062G]

(Mouse) Rnf2 Antibody (Center) [APR33062G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H, M

Specificity: This mouse Rnf2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 165-199 amino acids from the Central region of mouse Rnf2.

Cellular Localisation: Nucleus. Cytoplasm. Chromosome. Note=Enriched on inactive X chromosome (Xi) in female trophoblast stem (TS) cells as well as differentiating embryonic stem (ES) cells (PubMed:12183370) . The enrichment on Xi is transient during TS and ES cell differentiation. The association with Xi is mitotically stable in non-differentiated TS cells (PubMed:12183370) .

Molecular Weight: 37623

Clone: Polyclonal

Gene Name: Rnf2

Gene ID: 19821

Function: E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A (H2AK119Ub), thereby playing a central role in histone code and gene regulation (PubMed:15525528, PubMed:22325148, PubMed:28596365) . H2AK119Ub gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals (PubMed:15525528, PubMed:28596365) . May be involved in the initiation of both imprinted and random X inactivation (PubMed:15525528) . Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development (PubMed:22325148, PubMed:16710298) . PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility (PubMed:15525528, PubMed:22325148, PubMed:16710298) . E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4 (PubMed:16710298) . Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity (PubMed:15525528, PubMed:16710298) . Plays a role in the transcriptional repression of genes that are required for pluripotency in embryonic stem cells, thereby contributing to differentiation of the ectodermal and endodermal germ layers (PubMed:22226355) . Association with the chromosomal DNA is cell-cycle dependent. In resting B- and T-lymphocytes, interaction with AURKB leads to block its activity, thereby maintaining transcription in resting lymphocytes (PubMed:24034696) . Also acts as a negative regulator of autophagy by mediating ubiquitination of AMBRA1, leading to its subsequent degradation (PubMed:24980959) .

Summary: Tissue Location: Expressed in embryonic stem cells.

Form: N/A

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000 WB--1:2000

Synonyms: E3 ubiquitin-protein ligase RING2, 632-, RING finger protein 1B, RING1b, RING finger protein 2, Rnf2, DinG, Ring1b