Mouse S100 Calcium Binding Protein A8 (S100A8) ELISA Kit | KTE70488

Mouse S100 Calcium Binding Protein A8 (S100A8) ELISA Kit | KTE70488 | Gentaur UK, US & Europe Distribution

Application: This Mouse S100 Calcium Binding Protein A8 (S100A8) ELISA Kit employs a two-site sandwich ELISA to quantitate S100A8 in samples. An antibody specific for S100A8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyS100A8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100A8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100A8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse S100 Calcium Binding Protein A8 microplate
• Mouse S100 Calcium Binding Protein A8 standard
• Mouse S100 Calcium Binding Protein A8 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse S100 Calcium Binding Protein A8 (S100A8) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse S100A8. No significant cross-reactivity or interference between Mouse S100A8 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: S100A8, a protein associated with a wide variety of inflammatory conditions, is heavily over-expressed in association with inflammation. Human S100A8 possesses neutrophil-repelling properties that result in an anti-inflammatory effect in vivo. The chemotactic activity of S100A8 on neutrophils was tested in Transwell chemotaxis assays. Analysis of the data indicates that S100A8 causes a repulsion of peripheral neutrophils, an activity that S100A8 loses upon its oxidation. S100A8 causes a strong anti-inflammatory effect in the rat air-pouch model of inflammation in vivo. These data highlight a naturally occurring novel anti-inflammatory pathway and provide potential molecular targets for the development of novel anti-inflammatory therapeutics.

Alternative Names: S100A8; 60B8AG; CAGA; CFAG; CGLA; CP-10; L1Ag; MA387; MIF; MRP8; NIF; P8; OTTHUMP00000015330; S100 calcium-binding protein A8; S100 calcium-binding protein A8 (calgranulin A) ; calgranulin A; cystic fibrosis ant

Search name: S100A8; 60B8AG; CAGA; CFAG; CGLA; CP-10; L1Ag; MA387; MIF; MRP8; NIF; P8; OTTHUMP00000015330; S100 calcium-binding protein A8; S100 calcium-binding protein A8 (calgranulin A) ; calgranulin A; cystic fibrosis ant

Tag: S100A8