Mouse S100 calcium binding protein B (S-100B) ELISA Kit | AE20883MO

Mouse S100 calcium binding protein B (S-100B) ELISA Kit | AE20883MO | Gentaur UK, US & Europe Distribution

Species Reactivity: Mouse (Mus musculus)

Abbreviation: S-100B

Alternative Name: NEF; S100; S100beta; OTTHUMP00000174958|S-100 calcium-binding protein; beta chain|S100 beta|S100 calcium binding protein; beta (neural) |S100 calcium-binding protein; beta|S100 calcium-binding protei

Application: ELISA

Range: 31.25-2000 pg/mL

Sensitivity: 12.5 pg/mL

Intra-Assay: ≤3.8%

Inter-Assay: ≤7.2%

Recovery: 0, 96

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate S-100B in samples. An antibody specific for S-100B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyS-100B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S-100B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S-100B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: S100 proteins are localized in the cytoplasm and nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100B is glial-specific and is expressed primarily by astrocytes, but not all astrocytes express S100B. It has been shown that S100B is only expressed by a subtype of mature astrocytes that ensheath blood vessels and by NG2-expressing cells. This protein may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. In the developing CNS it acts as a neurotrophic factor and neuronal survival protein.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .