Mouse S17aH (17-Alpha-Hydroxylase) ELISA Kit | ELK7512

Mouse S17aH (17-Alpha-Hydroxylase) ELISA Kit | ELK7512 | Gentaur UK, US & Europe Distribution

Alternative Names: CYP17A1; CPT7; P450C17; 17-alpha-hydroxyprogesterone aldolase/17, 20-Lyase; Steroid 17-alpha-monooxygenase; Cytochrome P450, Family 17, Subfamily A, Polypeptide 1

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.58 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;Gastroenterology;Nutrition metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 17-Alpha-Hydroxylase (S17aH) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 17-Alpha-Hydroxylase (S17aH) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 17-Alpha-Hydroxylase (S17aH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 17-Alpha-Hydroxylase (S17aH) in the samples is then determined by comparing the OD of the samples to the standard curve.