Mouse Secretory immunoglobulin A ELISA Kit | KTE70438

Mouse Secretory immunoglobulin A ELISA Kit | KTE70438 | Gentaur UK, US & Europe Distribution

Application: This Mouse Secretory immunoglobulin (AsIgA) ELISA Kit employs a two-site sandwich ELISA to quantitate sIgA in samples. An antibody specific for sIgA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anysIgA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sIgA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sIgA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Secretory immunoglobulin microplate
• Mouse Secretory immunoglobulin standard
• Mouse Secretory immunoglobulin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Secretory immunoglobulin (AsIgA) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse sIgA. No significant cross-reactivity or interference between Mouse sIgA and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Immunoglobulin A (IgA, also referred to as sIgA) is an antibody that plays a crucial role in the immune function of mucous membranes. IgA has two subclasses (IgA1 and IgA2) and can be produced as a monomeric as well as a dimeric form. The IgA dimeric form is the most prevalent and is also called secretory IgA (sIgA) . SIgA is the main immunoglobulin found in mucous secretions, including tears, saliva, sweat, colostrum and secretions from the genitourinary tract, gastrointestinal tract, prostate and respiratory epithelium. It is also found in small amounts in blood. The secretory component of sIgA protects the immunoglobulin from being degraded by proteolytic enzymes, thus sIgA can survive in the harsh gastrointestinal tract environment and provide protection against microbes that multiply in body secretions. SIgA can also inhibit inflammatory effects of other immunoglobulins.

Alternative Names: sIgA

Search name: sIgA

Tag: sIgA