Mouse Semaphorin-4D (SEMA4D) ELISA Kit | KTE70459

Mouse Semaphorin-4D (SEMA4D) ELISA Kit | KTE70459 | Gentaur UK, US & Europe Distribution

Application: This Mouse Semaphorin-4D (SEMA4D) ELISA Kit employs a two-site sandwich ELISA to quantitate SEMA4D in samples. An antibody specific for SEMA4D has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySEMA4D present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA4D is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA4D bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Semaphorin-4D microplate
• Mouse Semaphorin-4D standard
• Mouse Semaphorin-4D detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Semaphorin-4D (SEMA4D) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SEMA4D. No significant cross-reactivity or interference between Mouse SEMA4D and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: CD100 was first identified as a cell surface protein of resting T cells; previous studies had shown that it was involved in lymphocyte activation. Hall et al. (1996) cloned CD100 from a cDNA library of phytohemagglutinin-activated human T cells. Sequence analysis showed that CD100 encodes an 863-amino acid polypeptide containing a secretory signal sequence, a single transmembrane domain, an immunoglobulin (Ig) -like domain, and a conserved 500-amino acid sema domain. Thus, Hall et al. (1996) stated that CD100 is a member of the semaphorin family and the first semaphorin believed to be involved in the immune system. See SEMA3F and SEMA3B. Northern blot analysis revealed that CD100 is expressed primarily as a 4.5-kb message in a variety of tissues, both hematopoietic and nonhematopoietic.

Alternative Names: SEMA4D; XXyac-YM68G7.1; C9orf164; CD100; FLJ33485; FLJ34282; FLJ39737; FLJ46484; M-sema-G; MGC169138; MGC169141; SEMAJ; coll-4; OTTHUMP00000021622; OTTHUMP00000208950; OTTHUMP00000208951; OTTHUMP00000208957; se

Search name: SEMA4D; XXyac-YM68G7.1; C9orf164; CD100; FLJ33485; FLJ34282; FLJ39737; FLJ46484; M-sema-G; MGC169138; MGC169141; SEMAJ; coll-4; OTTHUMP00000021622; OTTHUMP00000208950; OTTHUMP00000208951; OTTHUMP00000208957; se

Tag: SEMA4D