Mouse Semaphorin-7A (SEMA7A) ELISA Kit | KTE70458

Mouse Semaphorin-7A (SEMA7A) ELISA Kit | KTE70458 | Gentaur UK, US & Europe Distribution

Application: This Mouse Semaphorin-7A (SEMA7A) ELISA Kit employs a two-site sandwich ELISA to quantitate SEMA7A in samples. An antibody specific for SEMA7A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySEMA7A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA7A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA7A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Semaphorin-7A microplate
• Mouse Semaphorin-7A standard
• Mouse Semaphorin-7A detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Semaphorin-7A (SEMA7A) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SEMA7A. No significant cross-reactivity or interference between Mouse SEMA7A and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: SEMA7A is a membrane-bound semaphorin that associates with cell surfaces via a glycosylphosphatidylinositol (GPI) linkage. SEMA7A is also known as the John-Milton-Hagen (JMH) blood group antigen, an 80-kD glycoprotein expressed on activated lymphocytes and erythrocytes. Czopik et al. (2006) found that T cells from immunized Sema7a -/- mice had increased proliferative responses to antigen that were not attributable to Sema7a deficiency on macrophages or dendritic cells. Sema7a -/- mice were prone to die at the onset of experimental autoimmune encephalomyelitis (EAE) and had higher clinical EAE scores compared with wildtype littermates. Delayed-type hypersensitivity responses were also enhanced in Sema7a -/- mice.

Alternative Names: SEMA7A; CD108; CDw108; H-SEMA-K1; H-Sema-L; JMH; MGC126692; MGC126696; SEMAK1; SEMAL; John Milton Hagen blood group H-Sema K1; sema domain; immunoglobulin domain (Ig) ; and GPI membrane anchor; (semaphorin) 7

Search name: SEMA7A; CD108; CDw108; H-SEMA-K1; H-Sema-L; JMH; MGC126692; MGC126696; SEMAK1; SEMAL; John Milton Hagen blood group H-Sema K1; sema domain; immunoglobulin domain (Ig) ; and GPI membrane anchor; (semaphorin) 7

Tag: SEMA7A