Mouse SERT (Serotonin Transporter) ELISA Kit | ELK6411

Mouse SERT (Serotonin Transporter) ELISA Kit | ELK6411 | Gentaur UK, US & Europe Distribution

Alternative Names: SLC6A4; SLC6-A4; HTT; 5-HTT; 5HTT; OCD1; hSERT; Solute Carrier Family 6, Member 4; Neurotransmitter Transporter, Serotonin; Sodium-dependent serotonin transporter

Species: Mouse

Assay Type: Sandwich

Sensitivity: 12.7 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serotonin Transporter (SERT) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Serotonin Transporter (SERT) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serotonin Transporter (SERT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Serotonin Transporter (SERT) in the samples is then determined by comparing the OD of the samples to the standard curve.