Mouse Spartin (SPG20) ELISA Kit | KTE70386

Mouse Spartin (SPG20) ELISA Kit | KTE70386 | Gentaur UK, US & Europe Distribution

Application: This Mouse Spartin (SPG20) ELISA Kit employs a two-site sandwich ELISA to quantitate SPG20 in samples. An antibody specific for SPG20 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPG20 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPG20 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPG20 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Spartin microplate
• Mouse Spartin standard
• Mouse Spartin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Spartin (SPG20) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SPG20. No significant cross-reactivity or interference between Mouse SPG20 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: SPG20 encodes a protein that contains a MIT (Microtubule Interacting and Trafficking molecule) domain. This protein may be involved in endosomal trafficking, microtubule dynamics, or both functions. Frameshift mutations associated with SPG20 cause autosomal recessive spastic paraplegia 20 (Troyer syndrome) . Troyer syndrome (SPG20) is a complicated type of hereditary spastic paraplegias (HSPs) . HSP is a category of neurological disorder characterized by spasticity and muscle weakness in the lower limbs.

Alternative Names: SPG20; KIAA0610; SPARTIN; TAHCCP1; spartin; trans-activated by hepatitis C virus core protein 1

Search name: SPG20; KIAA0610; SPARTIN; TAHCCP1; spartin; trans-activated by hepatitis C virus core protein 1

Tag: SPG20