Mouse Sphingosine-1-phosphate lyase 1 (SGPL1) ELISA Kit | KTE70440

Mouse Sphingosine-1-phosphate lyase 1 (SGPL1) ELISA Kit | KTE70440 | Gentaur UK, US & Europe Distribution

Application: This Mouse Sphingosine-1-phosphate lyase 1 (SGPL1) ELISA Kit employs a two-site sandwich ELISA to quantitate SGPL1 in samples. An antibody specific for SGPL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySGPL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SGPL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SGPL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Sphingosine-1-phosphate lyase 1 microplate
• Mouse Sphingosine-1-phosphate lyase 1 standard
• Mouse Sphingosine-1-phosphate lyase 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Sphingosine-1-phosphate lyase 1 (SGPL1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SGPL1. No significant cross-reactivity or interference between Mouse SGPL1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Sphingosine-1-phosphate (SPP) participates in the proliferative signal transduction pathways of mammalian cells. SPP synthesis is catalyzed by sphingosine kinase, and SPP degradation is catalyzed by SPP lyase (SPL) . The first SPL gene cloned, BST1 ('bestowed of sphingosine tolerance'), was isolated from budding yeast . A yeast mutant strain containing a deletion of the entire BST1 coding region is sensitive to the growth-inhibitory effects of D-erythro-sphingosine, due to its inability to degrade SPP. Spl, that has 59% sequence similarity to the BST1 gene product. They showed that this cDNA can functionally complement the yeast BST1 deletion, restoring a sphingosine-resistant phenotype. Northern blot analysis detected Spl expression in several mouse tissues, with the highest level found in the liver.

Alternative Names: SGPL1; FLJ13811; KIAA1252; SPL;

Search name: SGPL1; FLJ13811; KIAA1252; SPL;

Tag: SGPL1