Mouse SRGN (Serglycin) ELISA Kit | ELK7758

Mouse SRGN (Serglycin) ELISA Kit | ELK7758 | Gentaur UK, US & Europe Distribution

Alternative Names: PRG; PPG; PRG1; proteoglycan 1, secretory granule

Species: Mouse

Assay Type: Sandwich

Sensitivity: 20.14 pg/mL

Standard: 3000 pg/mL

Detection Range: 46.88-3000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serglycin (SRGN) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Serglycin (SRGN) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serglycin (SRGN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Serglycin (SRGN) in the samples is then determined by comparing the OD of the samples to the standard curve.