Mouse TBP2 (Thioredoxin Binding Protein 2) ELISA Kit | ELK2459

Mouse TBP2 (Thioredoxin Binding Protein 2) ELISA Kit | ELK2459 | Gentaur UK, US & Europe Distribution

Alternative Names: TXNIP; HHCPA78; THIF; VDUP1; Thioredoxin Interacting Protein; Vitamin D3-Upregulated Protein 1; Upregulated By 1, 25-Dihydroxyvitamin D-3

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.117 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thioredoxin Binding Protein 2 (TBP2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thioredoxin Binding Protein 2 (TBP2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thioredoxin Binding Protein 2 (TBP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thioredoxin Binding Protein 2 (TBP2) in the samples is then determined by comparing the OD of the samples to the standard curve.