Mouse TFF1 (Trefoil Factor 1) ELISA Kit | ELK5526

Mouse TFF1 (Trefoil Factor 1) ELISA Kit | ELK5526 | Gentaur UK, US & Europe Distribution

Alternative Names: BCEI; HP1.A; HPS2; PS2; PNR2; Breast Cancer, Estrogen-Inducible Sequence Expressed In; Breast cancer estrogen-inducible protein; Polypeptide P1.A

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.065 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Tumor immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Trefoil Factor 1 (TFF1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Trefoil Factor 1 (TFF1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Trefoil Factor 1 (TFF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Trefoil Factor 1 (TFF1) in the samples is then determined by comparing the OD of the samples to the standard curve.