Mouse TGF-β1 protein, His tag (Animal-Free) | PRP1017

Mouse TGF-β1 protein, His tag (Animal-Free) | PRP1017 | Gentaur UK, US & Europe Distribution

Product Category: Cytology

Application: Cytokines

Product Type: Cytokines (Animal-Free)

Sequence: Amino acid sequence derived from mouse TGFβ-1 (P04202) (Met1-Ser390) was expressed with a 6 His tag at the C-terminus.

Activity: N/A

protein Lenght: The recombinant human Mouse TGF-β1 protein consists of 91 amino acids and predicts a molecular mass of 42.7 KD. It migrates as an approximately 54, 41 and 15 KD band in SDS-PAGE under reducing conditions.

Preparation: HEK293 Cells

Purity: >95% as determined by SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4.

Kit Component: N/A

Features & Benefits : Endotoxin: < 1.0 EU per µg of the protein as determined by the LAL method.

Molecular Weight: 42.7 KD

Usage Note: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse TGF beta 1 protein using the buffer we provided not less than 100 µg/mL, which can then be further diluted to other aqueous solutions.

Storage Conditions: Lyophilized Mouse TGF-β1 protein, His tag (Animal-Free) should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.99% HSA or BSA) . Please prevent freeze-thaw cycles.

Shipping: The product is shipped at ambient temperature.

Background: TGF-β1 is a member of the transforming growth factor β (TGF-β) family. The transforming growth factor-β family of polypeptides are involved in the regulation of cellular processes, including cell division, differentiation, motility, adhesion and death. TGF-β1 positively and negatively regulates many other growth factors. It inhibits the secretion and activity of many other cytokines including interferon-γ, tumor necrosis factor-α and various interleukins. It can also decrease the expression levels of cytokine receptors. Meanwhile, TGF-β1 also increases the expression of certain cytokines in T cells and promotes their proliferation, particularly if the cells are immature. TGF-β1 also inhibits proliferation and stimulates apoptosis of B cells, and plays a role in controlling the expression of antibody, transferrin and MHC class II proteins on immature and mature B cells. As for myeloid cells, TGF-β1can inhibit their proliferation and prevent their production of reactive oxygen and nitrogen intermediates. However, as with other cell types, TGF-β1 also has the opposite effect on cells of myeloid origin. TGF-β1 is a multifunctional protein that controls proliferation, differentiation and other functions in many cell types. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Once cells lose their sensitivity to TGF-β1-mediated growth inhibition, autocrine TGF-β signaling can promote tumorigenesis. Elevated levels of TGF-β1 are often observed in advanced carcinomas, and have been correlated with increased tumor invasiveness and disease progression.

Alternative Names: TGF-beta-1; CED; DPD1; TGFB; TGF-b1; TGFB1; CEDLAP;latency-associated peptide; TGFbeta; TGF-beta 1 protein; transforming growth factor beta-1; TGF-β1; TGF beta1; TGFbeta 1; TGF-beta 1; TGFbeta

Search Names: TGF-beta-1; CED; DPD1; TGFB; TGF-b1; TGFB1; CEDLAP;latency-associated peptide; TGFbeta; TGF-beta 1 protein; transforming growth factor beta-1; TGF-β1; TGF beta1; TGFbeta 1; TGF-beta 1; TGFbeta

Tag: TGF-β1