Mouse TGFb2 (Transforming Growth Factor Beta 2) ELISA Kit | ELK5216

Mouse TGFb2 (Transforming Growth Factor Beta 2) ELISA Kit | ELK5216 | Gentaur UK, US & Europe Distribution

Alternative Names: TGF-B2; TGF-Beta2; G-TSF; LAP; Cetermin; Polyergin; Latency-associated peptide; BSC-1 cell growth inhibitor; Glioblastoma-derived T-cell suppressor factor

Species: Mouse

Assay Type: Competitive Inhibition

Sensitivity: 8.93 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.

Assay Lenght: 2h

Research Area: Cytokine;Infection immunity

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Transforming Growth Factor Beta 2 (TGFb2) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Transforming Growth Factor Beta 2 (TGFb2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transforming Growth Factor Beta 2 (TGFb2) in the samples is then determined by comparing the OD of the samples to the standard curve.