Mouse TGFbR2 (Transforming Growth Factor Beta Receptor II) ELISA Kit | ELK6823

Mouse TGFbR2 (Transforming Growth Factor Beta Receptor II) ELISA Kit | ELK6823 | Gentaur UK, US & Europe Distribution

Alternative Names: MFS2

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.112 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Beta Receptor II (TGFbR2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Transforming Growth Factor Beta Receptor II (TGFbR2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transforming Growth Factor Beta Receptor II (TGFbR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transforming Growth Factor Beta Receptor II (TGFbR2) in the samples is then determined by comparing the OD of the samples to the standard curve.