Mouse TGM6 (Transglutaminase 6) ELISA Kit | ELK7329

Mouse TGM6 (Transglutaminase 6) ELISA Kit | ELK7329 | Gentaur UK, US & Europe Distribution

Alternative Names: TGM3L; TGY; SCA35; Transglutaminase 3-Like; Spinocerebellar Ataxia 35; Transglutaminase Y; Protein-glutamine gamma-glutamyltransferase 6

Species: Mouse

Assay Type: Sandwich

Sensitivity: 6.6 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transglutaminase 6 (TGM6) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Transglutaminase 6 (TGM6) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transglutaminase 6 (TGM6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transglutaminase 6 (TGM6) in the samples is then determined by comparing the OD of the samples to the standard curve.