Mouse THBS3 (Thrombospondin 3) ELISA Kit | ELK6457

Mouse THBS3 (Thrombospondin 3) ELISA Kit | ELK6457 | Gentaur UK, US & Europe Distribution

Alternative Names: TSP3

Species: Mouse

Assay Type: Sandwich

Sensitivity: 25.3 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: Plasma.

Assay Lenght: 3.5h

Research Area: Hematology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thrombospondin 3 (THBS3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thrombospondin 3 (THBS3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thrombospondin 3 (THBS3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thrombospondin 3 (THBS3) in the samples is then determined by comparing the OD of the samples to the standard curve.