Mouse Tissue inhibitors of metalloproteinase 2 (TIMP2) ELISA Kit | KTE71418

Mouse Tissue inhibitors of metalloproteinase 2 (TIMP2) ELISA Kit | KTE71418 | Gentaur UK, US & Europe Distribution

Application: This Mouse Tissue inhibitors of metalloproteinase 2 (TIMP2) ELISA Kit employs a two-site sandwich ELISA to quantitate TIMP2 in samples. An antibody specific for TIMP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTIMP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIMP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIMP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Tissue inhibitors of metalloproteinase 2 microplate
• Mouse Tissue inhibitors of metalloproteinase 2 standard
• Mouse Tissue inhibitors of metalloproteinase 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Tissue inhibitors of metalloproteinase 2 (TIMP2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TIMP2. No significant cross-reactivity or interference between Mouse TIMP2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: TIMP2, is a member of the TIMP family. The proteins are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. In melanocytic cells TIMP2 gene expression may be regulated by MITF.

Alternative Names: TIMP2; CSC-21K; tissue inhibitor of metalloproteinase 2; tissue inhibitor of metalloproteinases 2

Search name: TIMP2; CSC-21K; tissue inhibitor of metalloproteinase 2; tissue inhibitor of metalloproteinases 2

Tag: TIMP2