Mouse TNF-α protein | PRP1113

Mouse TNF-α protein | PRP1113 | Gentaur UK, US & Europe Distribution

Product Category: Cytology

Application: Cytokines

Product Type: Cytokines

Sequence: Amino acid sequence derived from Mouse TNF-α isoform (P06804) (Leu80-Leu235) was expressed.

Activity: Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 25-100 pg/ml.

protein Lenght: The recombinant Mouse TNF-α protein consists of 157 amino acids and has a predicted molecular mass of 17 kDa.

Preparation: E. coli

Purity: >98 % as determined by SDS-PAGE

Formulation: Lyophilized from sterile PBS, pH 7.4.

Kit Component: N/A

Features & Benefits : Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.

Molecular Weight: 17 kDa

Usage Note: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse TNF-alpha protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Storage Conditions: Lyophilized Mouse TNF-α proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.13% HSA or BSA) . Please prevent freeze-thaw cycles.

Shipping: The product is shipped at ambient temperature.

Background: Tumor necrosis factor α (TNF-α), also known as cachectin and TNFSF2, is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes.

Alternative Names: TNFSF2; DIF; Necrosin; Cytotoxin

Search Names: TNFSF2; DIF; Necrosin; Cytotoxin

Tag: TNF-α