Mouse TNFRSF17 (Tumor Necrosis Factor Receptor Superfamily, Member 17) ELISA Kit | ELK4626

Mouse TNFRSF17 (Tumor Necrosis Factor Receptor Superfamily, Member 17) ELISA Kit | ELK4626 | Gentaur UK, US & Europe Distribution

Alternative Names: CD269; BCM; BCMA; B-cell maturation protein

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.101 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction;CD & Adhesion molecule;Tumor immunity;Gastroenterology;Autoimmunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17) in the samples is then determined by comparing the OD of the samples to the standard curve.