Mouse TNFSF13 (Tumor Necrosis Factor Ligand Superfamily, Member 13) ELISA Kit | ELK3766

Mouse TNFSF13 (Tumor Necrosis Factor Ligand Superfamily, Member 13) ELISA Kit | ELK3766 | Gentaur UK, US & Europe Distribution

Alternative Names: CD256; APRIL; TALL2; TRDL1; ZTNF2; A Proliferation Inducing Ligand; TNF And APOL Related Leukocyte Expressed Ligand 2; TNF-Related Death Ligand 1

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.265 ng/mL

Standard: 40 ng/mL

Detection Range: 0.63-40 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: CD & Adhesion molecule;Tumor immunity;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13) in the samples is then determined by comparing the OD of the samples to the standard curve.