Mouse Transmembrane 4 L6 family member 1 (TM4SF1) ELISA Kit | KTE70203

Mouse Transmembrane 4 L6 family member 1 (TM4SF1) ELISA Kit | KTE70203 | Gentaur UK, US & Europe Distribution

Application: This Mouse Transmembrane 4 L6 family member 1 (TM4SF1) ELISA Kit employs a two-site sandwich ELISA to quantitate TM4SF1 in samples. An antibody specific for TM4SF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTM4SF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TM4SF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TM4SF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Transmembrane 4 L6 family member 1 microplate
• Mouse Transmembrane 4 L6 family member 1 standard
• Mouse Transmembrane 4 L6 family member 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Transmembrane 4 L6 family member 1 (TM4SF1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TM4SF1. No significant cross-reactivity or interference between Mouse TM4SF1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: TM4SF1 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface antigen and is highly expressed in different carcinomas. The predicted peptide sequence was 202 amino acids long and contained 3 predicted NH2-terminal hydrophobic transmembrane regions which were followed by a hydrophilic region containing 2 potential N-linked glycosylation sites and a COOH-terminal hydrophobic transmembrane region.

Alternative Names: TM4SF1; H-L6; L6; M3S1; TAAL6; membrane component; chromosome 3; surface marker 1; transmembrane 4 superfamily member 1; tumor-associated antigen L6

Search name: TM4SF1; H-L6; L6; M3S1; TAAL6; membrane component; chromosome 3; surface marker 1; transmembrane 4 superfamily member 1; tumor-associated antigen L6

Tag: TM4SF1