Mouse Transmembrane inner ear expressed protein (TMIE) ELISA Kit | AE14162MO

Mouse Transmembrane inner ear expressed protein (TMIE) ELISA Kit | AE14162MO | Gentaur UK, US & Europe Distribution

Species Reactivity: Mouse (Mus musculus)

Abbreviation: TMIE

Alternative Name: DFNB6; transmembrane inner ear protein

Application: ELISA

Range: Request Information

Sensitivity: Request Information

Intra-Assay: ≤6.1%

Inter-Assay: ≤7.6%

Recovery: 1, 01

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate TMIE in samples. An antibody specific for TMIE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMIE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMIE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMIE bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: TMIE encodes a transmembrane inner ear protein. This gene is one of multiple genes responsible for recessive non-syndromic deafness (DFNB), also known as autosomal recessive nonsyndromic hearing loss (ARNSHL), the most common form of congenitally acquired inherited hearing impairment. TMIE gene is expressed in many human tissues and encodes a transcript of approximately 2.5 kb. Repeated RACE experiments on cDNA from human retina and various other tissues yielded an approximately 1.7-kb transcript; no other TMIE transcripts were identified. Sequence analysis predicted an intracellular N terminus, 2 transmembrane regions separated by an extracellular loop, and an intracellular C terminus.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .