Mouse Transmembrane protein 53 (TMEM53) ELISA Kit | AE14280MO

Mouse Transmembrane protein 53 (TMEM53) ELISA Kit | AE14280MO | Gentaur UK, US & Europe Distribution

Species Reactivity: Mouse (Mus musculus)

Abbreviation: TMEM53

Alternative Name: RP4-678E16.2; FLJ22353; NET4; novel DUF829 domain-containing protein

Application: ELISA

Range: Request Information

Sensitivity: Request Information

Intra-Assay: ≤5.2%

Inter-Assay: ≤7.9%

Recovery: 0, 98

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate TMEM53 in samples. An antibody specific for TMEM53 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM53 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM53 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM53 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: TMEM53 has no paralogs but is predicted to have many orthologs across eukaryotes. The secondary structure of TMEM53 is predicted to consist of alternating pairs of alpha helices and beta sheets.The function of TMEM53 is not fully understood. It contains a domain of unknown function, DUF829, which is approximately 240 amino acids long. This domain has not been found in proteins other than TMEM53 and its orthologs. Based on human and mouse EST profiles and a human tissue GEO profile, TMEM53 appears to be expressed ubiquitously at low levels in both normal and cancerous tissues.Transmembrane protein 53 has no paralogs. It does, however, have orthologs extending throughout eukaryotes, from primates to amoeba.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .