Mouse TSPAN30 (Tetraspanin 30) ELISA Kit | ELK3340

Mouse TSPAN30 (Tetraspanin 30) ELISA Kit | ELK3340 | Gentaur UK, US & Europe Distribution

Alternative Names: CD63; LAMP-3; ME491; MLA1; OMA81H; Melanoma 1 antigen; Granulophysin; Lysosomal-associated membrane protein 3; Ocular melanoma-associated antigen

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.063 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: plasma, tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;CD & Adhesion molecule;Hematology;Developmental science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tetraspanin 30 (TSPAN30) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Tetraspanin 30 (TSPAN30) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tetraspanin 30 (TSPAN30), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tetraspanin 30 (TSPAN30) in the samples is then determined by comparing the OD of the samples to the standard curve.